While H-NO1 (226EE) successfully transmitted full-fledged CWD to TgElk mice (226EE), albeit with incomplete attack rates, it failed to cause clinical prion disease in both Prnp.Cer.Wt (226QQ) and Prnp.Cer.138NN (226QQ) mice. This evidence concerns the gene PRNP and prion disease.