CD8+ T cells were isolated from their tumor tissues for subpopulation analysis (Figure S8A), and further categorized into 12 subgroups based on classical markers and common attributes of CD8+ T cells, including naïve, effector, cytotoxicity, exhaustion, and senescence (Figure 6E-F, Figure S7B, Table S4). The gene discussed is CD8A; the disease is neoplasm.