Top genes alternating with NB cell development included ATP5MC2, TYMS, PTTG1, GNB2L1, NEUROD1, and IF16. For example, ATP5MC2 directly regulates ATP synthesis, TYMS is involved in DNA synthesis 56, and PTTG1 accelerates the G1/S phase transition of NB cells 57; these three oncogenes were upregulated during the early to middle stages of development (lineage 2) and downregulated as lineage 1 developed. Here, TYMS is linked to neuroblastoma.