Ultimately, <i>in-vitro</i> experiments demonstrated that LST1, a key gene in the risk model, was enhanced in the macrophage in response to LPS, which was closely related to the decrease of macrophage survival rate, the enhancement of apoptosis and oxidative stress injury, and the imbalance of the M1/M2 phenotype.<h4>Conclusions</h4>This study constructed a cuproptosis-related risk model to accurately predict the prognosis of sepsis. The gene discussed is LST1; the disease is Sepsis.