Toward this end we analyzed mass cytometry data from 292 individuals with DS relative to 96 euploid controls and tested for potential differences in immune cell subpopulations, identified using FlowSOM45, within the DS cohort based on number of autoimmune/inflammatory disease diagnoses, ANA positivity, TPO positivity, and positivity for additional autoantibodies. This evidence concerns the gene BTG3 and Dravet syndrome.