Sarkosyl insoluble tau is composed of tau aggregates, the primary component of NFTs, which are correlated with disease progression and cognitive impairment.35,61,62 Using Western blot analysis, we evaluated the levels of phosphorylated insoluble tau (AT8) and total insoluble human tau (Tau12) compared to the levels of their non-aggregated Sarkosyl soluble counterparts. This evidence concerns the gene MAPT and Cognitive impairment.