NLRP3 and serum lipopolysaccharide activity: To this end, we treated mice with 50 mg k−1g UK5099 before peritoneal injection of LPS to induce endotoxemia, which induces NLRP3‐dependent IL‐1β release in vivo.[9, 10] Mice were sacrificed at 6 and 12 hours after LPS injection and proinflammatory cytokines production in the serum was determined.