Finally, 3 hub mRNAs (BOC, MLST8, and HMGCS2) were identified, and GSEA analysis revealed that they controlled a great deal of immune system responses and inflammatory pathways, including IL-5 production, MAPK signaling pathway, and JAK-STAT signaling pathway, indicating that BOC, MLST8, and HMGCS2 may play key roles in CGN development. The gene discussed is MLST8; the disease is childhood gender nonconformity.