Given the distinct functions of Hsp90 when nitrated at Y33 versus Y56, we sought to determine the spatial distribution of these different forms of nitrated Hsp90 in three-dimensional (3D) cell culture models of schwannoma cells, using antibodies that specifically recognize either Hsp90NY33 or Hsp90NY56 [[19], [20], [21]]. The gene discussed is HSP90AB1; the disease is schwannoma.