On the other hand, there was no statistically significant difference when comparing the frequency of EC and seminoma that show a positive reaction to the PD-L1 antibody among a total number of EC (N ═ 22) and seminoma (N ═ 30) cases, respectively (P ═ 0.2 for PD-L1 positivity in lymphocytes, P ═ 0.085 for PD-L1 positivity in tumor cells). This evidence concerns the gene CD274 and seminoma.