However, when seeded in a pro-invasive Collagen type 1 matrix (Collagen pre-polymerized at 26 °C), we observed invasive patterns that are similar to the primary tissue sections (Fig. 1A, B), including expression of Keratin 14 (K14) a basal maker for poorly differentiated tumors and the tumor invasive front HNSCC. Here, KRT14 is linked to head and neck squamous cell carcinoma.