In a time course analysis, compared to cells transfected with negative control (NC) siRNA, A549 cells transfected with si-GBP1P1 were able to promote IAV M1 protein production much more significantly at late times (24 and 36 h) than at early times (12 h) post-infection (Fig. 4A). The gene discussed is GBP1P1; the disease is infection.