PGK1 is a well‐known oncoprotein reported to be involved in the activation of PI3K/AKT signaling.[36, 37, 38] Increased expression of PGK1 can activate the PI3K/AKT signaling pathway in gastric cancer progression, leading to a poor prognosis.[39] In our study, overexpression of either TMEM52B‐P18 or TMEM52B‐P20 failed to increase the levels of phosphorylated AKT in HONE‐1 cells with PGK1 knockdown, followed by failure to accelerate cell proliferation, suggesting that the similarity of TMEM52B isoforms in promoting NPC growth may lie in PGK1‐mediated AKT activation. This evidence concerns the gene PGK1 and nasopharyngeal carcinoma.