PGK1 is an ATP‐generating enzyme that activates the AKT/mTOR pathway in non‐small cell lung cancer.[14] To determine whether PGK1 is involved in TMEM52B‐dependent AKT phosphorylation and oncogenic behaviors such as proliferation, migration, and invasion, we designed siRNA oligonucleotides targeting PGK1 and introduced them into HONE‐1, then overexpressed both of the TMEM52B isoforms. This evidence concerns the gene MTOR and lung cancer.