We finally established an in vivo pulmonary metastasis tumor model using male BALB/c nude mice, and observed that the LV‐TMEM52B‐P20 group formed significantly more tumor nodules in lung tissues compared with the LV‐TMEM52B‐P18 group, and that mice with LV‐TMEM52B‐P20 tumors showed a rapid loss of body weight and decreased survival time (Figure 4E–G). This evidence concerns the gene TMEM52B and neoplasm.