TP53 and neoplasm: Intriguingly, we also noted that two unique TP53 mutations, namely TP53:c.614A>G (p.Tyr205Cys) and TP53:c.578A>G (p.His193Arg), were present in the same microdissected tissue, squamous area 3 of the intervening mucosa with tumor (M5), suggesting that clonal expansion was not present in our samples.