ACSS2 and Anxiety: To further support this finding, behaviors tests were carried out to examine the depressive-related and anxiety-like behaviors including tail suspension test (TST), forced swim test (FST), sucrose preference test (SPT), open field test (OFT), and elevated plus maze (EPM) in CRS-exposure mice with hippocampal ACSS2 overexpression by stereotaxically injecting the adeno-associated virus (AAV).