ACSS2 and major depressive disorder: When we incubated DNA probe with the same amount of hippocampus lysate from depression mice fed with water or d-mannose, we found that CRS caused reduction in both PPARγ and ACSS2 bindings to DNA probe, while d-mannose administration reversed the reductions of ACSS2 and PPARγ binding to the DNA probe (Fig. 8C).