Therefore, formalin-fixed paraffin-embedded tumor samples of 56 HNSCC patients, who have undergone resection and adjuvant radiotherapy, were analyzed by multiplex immunohistochemistry focusing on the detailed phenotypic characterization and spatial distribution of DCs, CD8<sup>+</sup> T cells, and T-helper cell subsets in different tumor compartments. The gene discussed is CD8A; the disease is head and neck squamous cell carcinoma.