BAX and central nervous system cancer: Next, the western blot was used to detced the effect of siKIF4Aon glioma apoptosis, the observations found that the protein level of BAX (a pro-apoptotic factor) was increased and that the protein level of Bcl-2 (an anti-apoptotic factor) was decreased by siKIF4A treatment in glioma cell lines (U251 and LN229) (Supplementary Fig. 2J).