After WZ-3146 treatment of glioma cell lines (U251 and LN229), KIF4A mRNA and protein expression in these cells was measured by qRT‒PCR and Western blot analyses, and the results demonstrated that KIFA4 expression was strongly decreased by WZ-3146 treatment in glioma cell lines (U251 and LN229) (Fig. 5F-G). The gene discussed is KIF4A; the disease is central nervous system cancer.