KIF4A and central nervous system cancer: Next, MTT assays suggested that KIF4A overexpression reversed the inhibitory effect of WZ-3146 on proliferation in U251 glioma cell line (Fig. 5H), moreover, the western blot results confirmed that the KIF4A over-expression could reverse the effect of WZ-3146 on KIF4A protein expression in glioma (Supplementary Fig. 2I).