The frequencies of positive immune markers (CD4, CD8, PD1, PD-L1, T-cell immunoglobulin and mucin domain 3 (TIM3), and forkhead box protein 3 (Foxp3)) in the above tissues were quantified by AKOYA Opal Polaris 7 Color Manual IHC Detection Kit.<h4>Results</h4>A total of 50 female patients with luminal A BC were enrolled in this study. The gene discussed is CD8A; the disease is breast cancer.