To investigate if the differences between both models can be explained by the inflammatory environment in the AOM/DSS model, we employed another model of sporadic CRC, in which AOM-induced mutagenesis in combination with epithelial cell-specific Tp53 deletion results in invasive CRC (Neufert et al, 2021; Schwitalla et al, 2013) (Fig. EV4A). This evidence concerns the gene TP53 and colorectal carcinoma.