Since Δ133p53α knock-down is not possible without also knocking-down Δ133p53β/γ and Δ160p53α/β/γ, we stably overexpressed WT Δ133p53α in WT TP53 GBM cells (U87/A172), and mutant Δ133p53α R273H in R273H mutant TP53 GBM cells (SF268/SNB19). The gene discussed is TP53; the disease is glioblastoma.