We confirmed the presence of significant correlations of NOTCH3 p.R544C, FV-H1299R, MTHFR-C677T, MTHFR-A1298C, FII Prothrombin, FV-Cambridge, PAI1 4G/5G, and FXIII Val34Leu with several factors in patients with ischemic stroke. Here, NOTCH3 is linked to ischemic stroke.