In this study, we aim to further investigate the possible association of selected variations (NOS3 (rs2070744), NOS1AP (rs10494366), REN (rs11240688), PLA2G4A (rs1015710), TCF7L2 (rs4506565, rs4132670, and rs7917983), ADRB1(rs1801252 and rs1801253), and PTPRD (rs4742610, rs10739150, and rs12346562) with hypertension in the Jordanian population and the effect on drug response and clinical symptoms. This evidence concerns the gene NOS3 and hypertensive disorder.