In the VEGFA gene, heterozygous substitution and one homozygous substitution in the mutation variants (905C>CG and 905C>G) on chromosome position (6:43738350) has occurred in GBM patients, and this mutation has been previously described; its variant percentage was 37.5%; while, the G→GA substitution with mutation variant (841G>GA) that occurred on chromosome position (6:43738173), in which this mutation was not found previously in the databases, and its variant percentage was 12.5%. This evidence concerns the gene VEGFA and glioblastoma.