In order to further elucidate the effect of HST on the viral infection outcome in rare minnows, the mRNA levels of Hsp70, IL-1β, MyD88 and NF-κB in rare minnows were systematically monitored following GCRV challenge at Day 0, 2, 4, 6, 8 and 10 p.i. (Figure 9). Here, NFKB1 is linked to viral infectious disease.