MT-RNR2 and infection: Interestingly, although western blotting analysis revealed the highest amount of SeV-HN protein in LV particles utilizing 3.0 μg of khcHN plasmids, its infectivity was significantly reduced compared with LVs utilizing 0.3 μg of khcHN plasmids, indicating that a higher level of expression of SeV-HN protein on the viral particle does not increase the infection efficiency (Figure 3C,D).