There were 467 dinucleotides with abnormal methylation status in NASH patients, and eight genes related to metabolism and the onset of NAFLD, including GALNTL4, ACLY, IGFBP2, PLCG1, PRKCE, IGF1, IP6K3, and PC [28]. The gene discussed is PRKCE; the disease is metabolic dysfunction-associated steatotic liver disease.