Selectivity assays over HEK293 cells expressing many different ion channels (KCNT2, Slo, GIRK1/2, Kv2.1, TREK1, hERG, NaV1.7, and Cav3.2) were performed, along with the evaluation of the activity of the initial hit selection over the epilepsy-related KCNT1 mutants G288S and R428Q. The gene discussed is KCNT1; the disease is epilepsy.