The two time points allow us to detect the clear changes in the motor function, SOCS3 levels, astrogliosis, and neuronal loss, which provide critical insights into the dynamic SOCS3 levels as ALS progressed and a reasonable rationale to further investigate the SOCS3 levels in the late symptomatic stage and even the disease endpoint in the future. Here, SOCS3 is linked to amyotrophic lateral sclerosis.