While we find that SOCS3 expression is significantly increased in the preBötC of ALS mice based on its unique anatomic location, future studies incorporating specific markers, such as Neurokinin-1 Receptor (NK1R), Somatostatin (SST), ChAT, and Paired-Like Homeobox 2B (PHOX2B), are needed to more accurately distinguish the preBötC from Nucleus Ambiguus. The gene discussed is SOCS3; the disease is amyotrophic lateral sclerosis.