SOCS3 and amyotrophic lateral sclerosis: Of most importance, the NeuN+ neurons were significantly decreased in the brainstem, especially in the preBötC of the brainstem in ALS mice at the pre-symptomatic stage (Figure 3E,F,K,N), and became even more exacerbated at the early symptomatic phases (Figure 3H,I,L,N), which was negatively related to the upregulated SOCS3 and was associated with neurodegeneration progression in a time-dependent manner.