Next, we analyzed metabolic signatures in plasma from (i) leukemia-bearing mice (NOTCH1-T tumors); (ii) mice injected with Lin-negative murine hematopoietic progenitors transduced with an empty vector (MiGRI) that do not develop leukemia (non-leukemic mice (NLM), at two time points post transplantation: early (2–3 weeks) and late (8–10 weeks); and (iii) mice with an abnormal but non-tumorigenic polyclonal CD4+CD8+ DP subset (pre-leukemia) at 2–3 weeks of transplantation. This evidence concerns the gene NOTCH1 and leukemia.