This study also showed that both cleaved capase-3 and phosphorylated MLKL (mixed lineage kinase domain-like protein), which increased under ischemia/reperfusion, were reduced by VBIT12, suggesting that not only do both apoptosis and necroptosis occur in glaucoma–ischemia models, but that both these processes are mediated by VDAC oligomerization. Here, VDAC1 is linked to ischemia.