Next, to investigated how necroptosis affects HNSCC progression in vivo, we transfected SCC25 and FaDu cells with tet-on lentivirus (Fig. S1E) containing phospho-mimetic MLKL (T357E/S358D) [33] and generated two doxycycline-inducible necroptosis cell lines (MLKL-25, MLKL-FD). The gene discussed is MLKL; the disease is head and neck squamous cell carcinoma.