Via qRT‒PCR, we analysed and confirmed the expression of 9 genes involved in BC-related pathways, CTNNB, FGF2, GNB4, CDH2, FGFR1, CHRM3, ITGA9, SGK1 and COL4A2 (Supplementary material 1, Supplemental data, Figure S6, Panels A‒I), validating the results of the RNA‒Seq analysis. The gene discussed is CTNNB1; the disease is breast cancer.