Via qRT‒PCR, we analysed and confirmed the expression of 9 genes involved in BC-related pathways, CTNNB, FGF2, GNB4, CDH2, FGFR1, CHRM3, ITGA9, SGK1 and COL4A2 (Supplementary material 1, Supplemental data, Figure S6, Panels A‒I), validating the results of the RNA‒Seq analysis. This evidence concerns the gene CHRM3 and breast cancer.