AKT1 and cancer: Moreover, the identification of pathways found to be deregulated in O-PRE vs. O-POST by transcriptomic analysis was further corroborated by performing a pseudobulk approach on the scRNA-Seq data, where GSEA confirmed the activation of the “PI3K-Akt signalling pathway”, “Wnt signalling pathway”, “Focal adhesion”, “Pathways in cancer” and “ECM-receptor interaction” pathways (Supplementary Material 1: Figure S7).