Therefore, we conducted a two-step MR study to comprehensively assess the mediating role of different iron homeostasis biomarkers in the associations of genetic liability to T2D and glycemic traits in liver steatosis, liver cirrhosis and its biomarkers (ALT and magnetic resonance imaging (MRI)-derived proton density fat fraction (PDFF)) [22, 23]. This evidence concerns the gene GPT and cirrhosis of liver.