CD3- and CD4- tumors were significantly associated with advanced stage (p < 0.001), high T stage (T3/T4, (p < 0.001)), positive lymph node metastasis (p < 0.001), poor tumor differentiation (p = 0.004) and presence of PNI (CD3: p = 0.006, CD4: p = 0.001), LVI (CD3: p = 0.02, CD4: p < 0.001) and BI (CD3: p < 0.001, CD4: p = 0.02) compared to CD3 + and CD4 + tumors respectively (Table 3). This evidence concerns the gene CD4 and neoplasm.