Subsequently, mapping the longevity-associated DEGs of MDD on the Longevity regulating pathway (map04211) indicated that the identified DEGs participate in the Insulin signaling pathway, PI3K-Akt signaling pathway, mTOR signaling pathway, FOXO signaling pathway, and AMPK signaling pathway (see Supplementary File 1: Fig. 4). This evidence concerns the gene MTOR and major depressive disorder.