Further analysis of 89 cell clusters showed that activated CD8+ T cell cluster (CD8T_06_CD69) and five DC subtypes, including DC_01_CLEC9A and DC_02_CD1C, were also decreased in LNM-ICC (Fig. 1E and Supplementary Fig. S1A). The gene discussed is CD8A; the disease is intrahepatic cholangiocarcinoma.