Gene Set Enrichment Analysis (GSEA) of RNA-seq data from FU-iCCA cohort showed that Hallmark gene sets like MTORC1 signaling, Glycolysis, Hypoxia, WNT/β-catenin signaling, and Reactome pathway TCF-dependent signaling in response to WNT were enriched in ICC samples with LNM (Supplementary Fig. S4A, B). This evidence concerns the gene HNF4A and intrahepatic cholangiocarcinoma.