Differentially expressed gene analysis of single malignant cells in SC-iCCA cohort also revealed reduced CXCL12 gene expression in LNM-ICC, as well as CCL4 and CCL5 (Supplementary Fig. S3A–C), which were reported to be DC chemoattractants [23, 24]. This evidence concerns the gene CCL4 and intrahepatic cholangiocarcinoma.