To identify GLI2 downstream genes responsible for the anti-ESCC activity of Tan, we integrated these genes including the 587 genes with a significant variation following Tan treatment in TE-1 cells (S1 Table), the differentially expressed genes in ESCC tissues and normal esophageal tissues using the TCGA database (S3 Table), and the downstream target genes of GLI2 using hTF-target database (S4 Table). This evidence concerns the gene GLI2 and esophageal squamous cell carcinoma.