Interestingly, the antiproliferative effects of grapefruit-derived nanovesicles on the A375 human melanoma cell line have also been correlated to Akt phosphorylation inhibition [31], as opposed to the stimulation of Akt phosphorylation by citrus-derived EVs and its correlation to the induction of human skin fibroblast proliferation observed in the present study. The gene discussed is AKT1; the disease is melanoma.