Next, in the ANOVA model(s), we entered an interaction term “tissue (0 = healthy, 1 = BCC) x nonsynonymous mutation in PTCH1 (0 = no mutation, 1 = mutation)” to find out the genes that had a different magnitude of differential expression in the BCC tissue in the absence or presence of the mutation. This evidence concerns the gene PTCH1 and skin basal cell carcinoma.