Figure 4A shows representative western blots of the mentioned proteins. In HCC group, a decrease in DNMT1, DNMT3a, and UHRF1 nuclear expression, but not DNMT3b, is observed; in addition, a slight increase in DNMT1 acetylated form (DNMT1Ac; p < 0.05). However, in the PFD-treated group, a significant increase in DNMT1 expression (p < 0.001), DNMT3a (p < 0.0001), and UHRF1 (p < 0.0001), as well as DNMT1Ac (p < 0.00001) was observed. This evidence concerns the gene DNMT3A and hepatocellular carcinoma.