HLAIImaster was applied to analyze two datasets of personalized melanoma vaccine neoantigens (\documentclass[12pt]{minimal}\usepackage{amsmath}\usepackage{wasysym}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{amsbsy}\usepackage{upgreek}\usepackage{mathrsfs}\setlength{\oddsidemargin}{-69pt}\begin{document}$n=232$\end{document} in total) with experimentally wet-lab validation (in vitro CD4+ T lymphocyte enzyme-linked immunospot) [44, 45]. Here, CD4 is linked to melanoma.