To investigate the functional impact of HIF1A-AS2 on HNSCC progression, we generated two pairs of HNSCC cells overexpressing HIF1A-AS2 versus a control vector (SAS-HIF1A-AS2/SAS-Vec and OECM1-HIF1A-AS2/OECM1-Vec) and evaluated the malignant phenotype of the cancer cells. The gene discussed is HIF1A; the disease is head and neck squamous cell carcinoma.