Among IPA’s preconstructed pathways, the 14-3-3-mediated pathway, cancer drug resistance pathway, AMPK signaling pathway, adipogenesis pathway, 3-phosphoinositide biosynthesis pathway, aryl hydrocarbon receptor signaling pathway, 4-1BB signaling in T lymphocytes, and acute myeloid leukemia signaling pathway were all found to be significantly different between the two cell lines and potentially involved in Gem resistance (Table 3). This evidence concerns the gene GEM and acute myeloid leukemia.