TP53 and colorectal carcinoma: To establish a mouse model of orthograde CRC metastasis we used murine CRC tumor organoids deficient for Apc, Trp53 and Tgfbr2 and that display a Kras gain of function mutation (G12D mutant) together with a constitutively activated Akt1 (hereafter referred to as APTAK organoids) (29).