To explore the specific pathophysiological relevance of RIPK1 and NSF binding to ASIC1a in the context of ischemic brain injury, we assessed the expression levels of RIPK1 and NSF in the sham-operation or the peri-infarct region of ipsilateral brain, 2 h following sham-operation/transient middle cerebral artery occlusion. The gene discussed is RIPK1; the disease is brain injury.