As a control, we used AAV- EF1α-CasRx that does not contain gRNA of Ripk1 and Nsf. Stroke size, edema and neurological deficits were assessed using the Bederson score, RotaRod test, and Adhesive removal test to determine the impact of Ripk1 and Nsf knockdown after tMCAO. This evidence concerns the gene RIPK1 and stroke disorder.