The principle of action of CAR-T cells is that their immune synapses bind to the antigenic targets on the surface of tumor cells, which in turn secretes substances such as perforin and granzyme, causing holes to be created in the cell membranes of the target tumor cells, and then the granzyme enters the target tumor cells through the holes, resulting in a chain reaction of cysteine-aspartate proteases that cause the tumor cells to lysate, and the CAR-T can autocrine release cytokines to promote its own activity and regulate the tumor microenvironment. The gene discussed is PRF1; the disease is neoplasm.