The study aimed to identify beta-thalassemia trait cases along with its clinical findings and hematological correlation through various methods of analysis of hematological parameters such as Hb, red blood cell (RBC) indices, Mentzer index, and red cell distribution width (RDW), high-performance liquid chromatography (HPLC) screening for HbA2/F levels [12]. This evidence concerns the gene HBA2 and Beta-thalassemia.