The beta-thalassemia trait in Wickramaratne KAC et al. was diagnosed mainly based on the HPLC screening with HbA2 levels between 3.5% and 7.5% were considered as beta-thalassemia trait and non-beta-thalassemia trait with HbA2 levels less than 3.5%. This evidence concerns the gene HBA2 and beta thalassemia.