Since, very few studies have been conducted related to evaluating role of chlorhexidine mouthwash on exacerbation of asthma in children and finding its significant clinical importance in modern dentistry, in the present study, we decided to investigate the effects of chlorhexidine mouthwash (the gold standard for maintaining oral hygiene) on serum interleukins 4, 5 and 13 in children. This evidence concerns the gene IL4 and asthma.