TLR signaling pathways were abundantly accumulated in Mac_C2 (Supplementary Fig. S3D), such as MyD88 deficiency (TLR2/4), IRAK4 deficiency (TLR2/4), regulation of TLR by endogenous ligand, and disease associated with the TLR signaling cascade, which were related to the inflammatory mechanisms of in cancers [28, 29]. This evidence concerns the gene IRAK4 and cancer.