Next, to investigate the change of proteome in PCa cells with an androgen-receptor inhibitor and for the less complexity of cell sample than tumor tissue, we used label-free quantification (LFQ) mass spectrometry to detect protein profiles in androgen receptor (AR)-positive LNCaP cells and AR-negative PC3 cells under enzalutamide treatment (Additional file 4: Figure S4A). This evidence concerns the gene AR and posterior cortical atrophy.